Digestion is an elementary process in fish metabolism because it determines the availability of nutrients needed for all biological concerns. Their energy requirements are derived from the oxidation of organic compounds (carbohydrates, lipids and proteins) produced from food digestion. The energy metabolism is the main and most important biological parameter involved in all physiological processes of lower vertebrates. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: All relevant data are within the paper and its Supporting Information files.įunding: This work was partly supported by the University of Reims Champagne-Ardenne and the financial support of the P190 Ecotoxicology Program of the French Ministry for Ecology and Sustainable Development.Ĭompeting interests: The authors have declared that no competing interests exist. Received: NovemAccepted: MaPublished: April 3, 2018Ĭopyright: © 2018 Hani et al. PLoS ONE 13(4):Įditor: De-Hua Wang, Institute of Zoology, CHINA (2018) Digestive enzymes and gut morphometric parameters of threespine stickleback ( Gasterosteus aculeatus): Influence of body size and temperature. However, a temporal variation was recorded for both RGM (in the second experiment) and ZI (in both experiments), and the later was correlated to fish body mass.Ĭitation: Hani YMI, Marchand A, Turies C, Kerambrun E, Palluel O, Bado-Nilles A, et al.
Temperature (in both experiments) had no effect on morphometric parameters. When sticklebacks were exposed to three constant temperatures (16, 18 and 21☌), a temporal effect correlated to fish growth was observed with inverse evolution patterns between amylase activity and the activities of trypsin and IAP. The highest levels of amylase and trypsin activity were observed at 18☌, while the highest IAP activity was recorded at 20☌.
When mimicking seasonal variation in temperature, body size had no effect on digestive enzyme activity. We described for the first time (i) three digestive enzymes: amylase, trypsin and intestinal alkaline phosphatase (IAP), and (ii) three gut morphometric parameters: relative gut length (RGL), relative gut mass (RGM) and Zihler’s index (ZI) in threespine stickleback ( Gasterosteus aculeatus), and we studied the effect of temperature and body size on these parameters. Determining digestive enzyme activity is of potential interest to obtain and understand valuable information about fish digestive physiology, since digestion is an elementary process of fish metabolism.
0 kommentar(er)
